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Technical Platform
Using innovative staining technics and professional IT equipment, neuron experts is capable to undertake the following services on cultures according to client needs:
Neuroprotective effect after drug treatments
Neuron survival (counting, LDH, MTT);
Neurite outgrowth;
Phenotype evaluation;
Neurotransmitters concentration;
Apoptosis evaluation...
Neurotrophic effect after drug treatments
Neurite outgrowth;
Neuronal maturation;
Synapse, vesicles evaluation (number of synapses);
Functionality of cells after drug treatments;
Electric activities;
Ca2+ measure;
Contraction activity (for co culture nerve muscle);
Neurotransmitters concentrations, synaptic vesicles measure…
For every studies, a positive (and an optional negative) control using reference compound (based on literature or in clinical evaluation) is used. Regularly, neuron experts develops new technics involving technical platforms available at the Marseille university (Faculte de Medecine Nord de Marseille, Institut Jean Roche) in France.
Jean Roche Institute available technical plateforms:
Confocal Microscopy (multiphotonic excitation), Electronic Microscopy, Picture analysis, Flux cytometry, cellular
screen, High sensitivity scan Gnome, Ca2+ flow measure…
Proteomic : Biacore 3000, (to analyze synaptic vesicles in neuronal cells and their neurotransmitters
Transgenesis center : CTA, animal facilities for Tg animal;
UMAG….
