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Neuroprotective effect after drug treatments
 Neuron survival (counting, LDH, MTT)
Neurite outgrowth, synaptogenesis
Phenotype evaluation (receptor,
channel, membrane proteine
evaluation,...)
Neurotransmitters concentration
Apoptosis evaluation (caspase, Bcl2,
Bax, Bad, ...)Mitochondria evaluation, translocation
evaluation
Neurotrophic effect after drug treatments
Neurite outgrowth
Neuronal maturation
Synapse, vesicles evaluation (number
synapses)Neuronal activity
Functionality of cells after drug treatments
Electric activities
Ca2+ measure
Contraction activity (for co- culture
nerve muscle)
Neurotransmitters concentrations,
synaptic vesicles measure…
Enzymatic evaluation
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Multiple sclerosis models
Oligodendrocytes-neurons co-culture
with BBB
Progressive injury of the BBB
Neuromuscular junction disease models (myasthenia, dystrophy, myopathy…)
Spinal cord (mdx, transgenic
animals…) co-culture
Optic nerve lesion models (optical neuropathy, ischemia…)
Retinal cells culture, ganglion cell
culture…
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Isolated cell culture
Cortical neurons
Hippocampal neurons
Mesencephalic dopaminergic neurons (striatum and substantia nigra)
Mesencephalic GABAergic neurons
Cerebelum neurons
Spinal cord motoneurons
Sensory neurons (DRG)
Glial cells (astrocytes, oligodendrocytes, Schwann cells, fibroblasts)
Muscle cells (myoblasts, myotubes,
innervated myofibres)
Co-cultures
Neurons / glial cells (astrocytes, oligodendrocytes, Schwann cells)
Neurons / muscular cells
Neurons / neurons
Organotypic cultures
DRG (dorsal root ganglia)
Spinal cord explants
Neuronal junction (nerve muscle co-culture)
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| Dopa neurons |
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Cortical neurons |
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Spinal Cord motoneurons |
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DRG neurons |
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Alzheimer’s models
Astrocytes / cortex or hippocampus neurons co-culture intoxicated with ß-amyloid
fragment (1-40, 1-42 or 25-35 fragments)
Astrocytes / cortical or hippocampal neurons co-culture intoxicated with glutamate or N
methyl-D-aspartate (NMDA)
Parkinson’s models
Astrocytes / Mesencephalic dopaminergic neurons intoxicated with MPP+ or Rotanone
Ischemia models
Oxygen deprivation on cortex / hippocampal neurons
Glutamate, H2O2 intoxication
Multiple sclerosis and demyelinated diseases models
Oligodendrocytes / motoneurons co-culture
Myelination models (DRG/Schwann cells)
Peripheral neuropathies models
Schwann cells / Sensory neurons intoxicated by toxic agents (vincristine, cisplatin, taxol,
Forskolin, pyridoxine and anti-viral agents)
Motoneurons intoxicated by toxic agents (vincristine)
Motoneuron disease models
Astrocytes / motoneurons co-culture
Spinal cord organotypic culture intoxicated with NMDA
Neuropathic pain models
DRG neurons hyper-sensitization of vanilloid receptor, Na+, K+ channels
Sensory neurons under inflammatory process
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CONFOCAL MICROSCOPY |
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